ABSTRACT
To establish a method for simultaneous determination of the contents of sesamoside, menthyl chloride, chlorogenic acid, caffeic acid, 8-O-acetyl-phloridzin methyl ester, forsythiaside B, luteolin, and ergosterol, the LIPLC analysis was performed on a Kinetex XB-C₁₈ (4.6 mm×50 mm,2.6 μm)column with acetonitrile (A)-0.1% phosphoric acid solution (B) as the mobile phase. The flow rate is 1.0 mL min⁻¹. The detection wavelength was 238, 320 and 350 nm and column temperature was 30 ℃. The results showed that the eight components could be completely separated with good linear relationships (r≥0.999 8) in the linear range. The average recoveries were 96.61%-100.4%. The method was simple with strong specificity and high precision. It can be used for the identification and quality evaluation of the Lamiophlomis rotate.The results also showed that there was a correlation between the various components, and the habitat was the main factor affecting the content of the eight components. However, the degradation gradient had no obvious effect on the contents, while the content of sesamoside was significantly decreased after one year of storage.
ABSTRACT
Objective: To study the chemical constituents from the whole plant of Orobanche coerulescens. Methods: The constituents were isolated and purified by using various column chromatographic techniques including D101, silica gel, and ODS, and the structures were identified by spectroscopic analyses including HR-ESI-MS, NMR, and 2D-NMR. Results: A new phenethyl alcohol glycoside was isolated from 50% ethanol extract in the whole plant of O. coerulescens, and its structure was identified as 2-(3-methoxy-4-hydroxy)-phenyl-ethanol-1-O-α-L-[(1→3)-4-O-acetyl-rhamnopyranosyl-4-O-feruloyl]-O-β-D-glucopyranoside. Conclusion: Compound 1 is a new compound named as orobancheoside A.